| α/α | Twinned City | Decision of the Municipal Council Council Municipality of Lefkada | Date of Twinning | Full name Mayor's Office |
| 1 | Swedish automobiles Emmaboda | 22/4-2-1985 | 18 August 1985 | Spyros Margelis |
| 2 | Strangice Czech Republic Straznice | 27/9-2-1987 | 15 June 1988 | Spyros Margelis |
| 3 | Paralimni Cyprus Paralimni | 47/6-3-1989 | 20 August 1989 | Spyros Margelis |
| 4 | Shinjuku Japan Shinsuku | 86/13-5-1985 | 12 October 1989 | Spyros Margelis |
| 5 | Nahariya Israel Naxarigia | 19/20-1-1992 | 16 August 1992 | Spyros Margelis |
| 6 | Ploiesti Romania Ploesti | 110/28-7-1997 | 28 April 1998 | Costas Stamatelos |
| 7 | Lecate France Leucate | 232/4-12-2000 | 22 September 2001 | Panagiotis Hardy |
| 8 | Fork Primorskiy Municipality of Odessa Municipality of Ukraine | 2/3-2-2010 | 4 September 2010 | Basil Fetsis |
| 9 | Zhoushan China | 12/19-1-2015 | 14 June 2015 | Konstantinos Drakontaidis |
